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Èçâåñòèÿ Íàöèîíàëüíîé àêàäåìèè íàóê Áåëàðóñè ÑÅÐÈß ÌÅÄÈÊÎ-ÁÈÎËÎÃÈ×ÅÑÊÈÕ ÍÀÓÊ Èçäàòåëü Áåëàðóñêàÿ íàâóêà, Ìèíñê, Ðåñïóáëèêà Áåëàðóñü |
Summary: Same characteristics of ECG of students before and after the decision of visual-motor tasks were explored Before the assembling of puzzle the anxienty was defined There were two groups of subjects the first with normal binocular vision and the second with monocular vision It were shown the connections between dynamics of the heart rhythm, monocular or binocular visions, degree of the anxienty and the level of the achievement in visual-motor tasks.
Azev O. A., Soltanov V. V.
Electrophysiological analysis of conduction pathways of interoceptive signals to neurons of vestibular nuclei in rats. pp. 14--17
Summary: In experiments on anesthetized Wistar rats, it was found that spontaneous modulation of neuron activity of vestibular nuclei by intestinal and gastric rhythms as well as that induced by distention of the small intestine persisted after subdia-phragmatic vagotomy and was inhibited after novocain block of bundles of the superior mesenteric plexus or after transection of the spinal cord at T5-T7. It is concluded that afferent signals from the stomach and intestine run to vestibular bulbar nuclei along mesenteric nerves, celiac plexus, and ascending spinal pathways.
Beloenko E. D., Ilyasevich I. A., Skakun P. G., Soshnikova E. V., Kandybo I. V.
Mechanisms of neuro-muscular and vascular changes in patients with habitual shoulder dislocation. pp. 18--23
Summary: 30 patients with habitual shoulder dislocation and 10 healthy control subjects have been studied by electophysiological methods (EMG/EP, RVG). The electrophysiological recodings revealed some changes in neuromuscular and vascular systems of upper limbs after the surgical treatment. The role of reflectory changes in developing the mechanisms of neuromuscular and vascular deficiency in chronic pathology in shoulder joint has been shown. It has been established that the effect of rehabilitation depends on the initial functional state of the neuromuscular and vascular systems. The electrophysiological prognostic criteria and terms of rehabilitation in patients with habitual shoulder dislocation have been determined.
Ilyasevich I. A., Beloenko E. D., Mazurenko A. N.
Neurophysiological criteria of the spinal cord conduction in patients with thoracic-lumbar complicated spine injury. pp. 24--32
Summary: The study deals with alterations of the corticospinal system after spinal cord injury. Four groups of 60 patients with thoracic-lumbar complicated spine injury were compared with a group of 10 healthy control subjects. Electrophysiological recodings (electromyographic-EMG, motor evoked potentials -- MEP and somato-sensory evoked potentials -- SSEP) allow the differentation between complete and partial loss of lower limbs function and spinal cord. In patients from groups I and II, voluntary muscle activity of lower limbs was preserved and the decrease in the amplitude of H-, M-potentials and MEP-recodings was 10 to 20 and 25 to 40%. In patients from group III with the loss of voluntary activity, the decrease in the amplitude of H-, M-potentials and MEP-recodigs was over 60% compared with the control group. Obviously, the intact morphology substance is insufficient for functional recovery. Complete spinal cord injury was diagnosed in patients from group IV. Thus, the early diagnostics and spinal cord function prognosis in patients from groups I, II and III with incomplete spinal cord preservation are of great importance in planning the rehabilitation program.
Zhukova I. A., Amvrosiev A. P.
Peculiarities of influence of prolonged small dose g-irradiation on state of hemocapillanes of white rat fetuses. pp. 33--36
Summary: The paper deals with study of ultrastructure reorganizations and morphofunctional changes of basic indices of testicular hemocapillaries of 20-day-old prenatal rat fetuses under conditions of prolonged external irradiation in
Summary: dose of 0.5 Gy (power 3.08 • 10-7 Gy/s). It was established that in period of prenatal development under conditions of prolonged external irradiation, significant changes in structure of hemocapillaries and in their endothelium cells existed. Later on, these changes can lead to breach of processes of development and functional formation of the organ.
Summary: In different periods after unilateral (left) neurotoxic (kainic acid, 1 mg/ml, 50 nl) destruction of the rostral nucleus of the solitary tract (NST), intraperitoneal application of lipopolisaccharide E coli (10 mg/kg, 5 h before decapitation) differ entially affected the activity of serotoninergic brainstem structures There was a marked activation of the serotonin metabolism in the hypothalamus, dorsomedial and caudoventral medulla 7 days after the lesion of NST and its attenuation 14 days after.
Yemelyanova A. A., Soltanov V. V.
Peculiarities of the ultrastucture of dorsal motor nucleus of the vagus nerve of medulla oblongata in rats in conditions of endotoxemia. pp. 41--44
Summary: Changes in the fine structure of neurons, their processes, sinapses, vessels of the dorsal nucleus of the vagus nerve in conditions of experimental endotoxemia were found. Possible mechanisms underlying these changes are discussed.
Summary: State of the hypophysial -- thyroid system during migration ascariasis was investigated. It was shown that ascaris larvas migration is accompanied with TTG, T3, and T4 secretion decrease. The host organism sensibilization with parasite antigens causes the similar alterations. The specific and pathogenetic therapy which was carried out on early stages of ascaris larvas migration causes the TTG, T3, T4 levels increase in comparison with infected animals.
Semak I. V., Korik E. O., Naumova M. V., Slominski A.
Interaction of products of peroxidase oxidation of mono-, di-, and trihydroxyflavones with glutathione. pp. 50--56
Summary: Ability of 3-hydroxyflavone, 5-hydroxyflavone, 7-hydroxyflavone, 3,7-dihydroxyflavone, 5,7-dihydroxyflavone, and 3,5,7-trihydroxyflavone to be oxidized by horseradish peroxidase and to conjugate with reduced gluthatione was studied using reverse phase chromatography and mass-spectrometry. It was determined that only flavonoids, having C3-OH group, undergo peroxidase oxidation. Products of peroxidase oxidation of these flavonoids interact with glutathione, forming monogluthatione adducts.
Vinogradova T. A., Vodoyevich V. P., Kovalchuk V. G. †, Slobodskaya N. S., Gurinovich V. A., Vinogradov V. V.
Rat blood hormonal profile and protective role of thiamine under immobilization stress. pp. 57--61
Summary: Cause of death of the animals in the «depletion» phase (72 h) was overproduction of stress hormones which in combination with insulin deficiency (trasitory stress diabetes) may induce myocardium damage incompatible with life. Thiamine decreased the amplitude of an adrenal steroidogenic reaction under immobilization stress and prevented death of the animals by switching the antistress mechanism of homeostatic insulin control.
Korik E. O , Naumova M. V., Slommski A., Semak I. V.
Possible mechanisms of formation of glutathione conjugates of quercetin and rutin. pp. 62--67
Summary: Comparative analysis of ability of products of peroxidative oxidation of rutin and quercetin to take part in reactions of conjugation with glutathione was carried out. It was shown that glutathione adducts of quercetm and rutin are different. The next schemes of formation of conjugates are suggested. Semiquinone that is a product of peroxidative oxidation of quercetm disproportionates directly into quinone methide, which cooperate with glutathione forming glutathione adducts at C6 and C8 positions of the ring A. Blocking of oxygen in C3 position of rutm by disaccharide prevents qumone methide isomerisation and limits formation of glutathione adducts by B-ring of o-quinone of flavonoid.
Kirillov V. A., Stebenyaeva E. E., Paplevka A. A., Demidchik E. P.
Thyroid cancer diagnosis using mor-phometric assessment of changes in lymphoid cell population of thyroid gland tissue. pp. 68--75
Summary: System of criteria has been developed, which is based on a set of boundary values of quantitative features reflecting the regularities of morphometric changes in the population of cells in the lymphoid series of the thyroid tissue with malignant and benign pathology. Weighting coefficients included into the system of features have been assessed, and the principle for calculation of the diagnostic index has been presented, which made it possible to differentiate thyroid diseases of malignant and benign nature by noninformative punctate smears.
Makarchikov A. F., Rusina I. M.
Isolation, purification, and physicochemical and kinetic properties of minor form of thiamine triphosphatase in bovine kidneys. pp. 76--79
Summary: A modified form of cytosolic thiamine triphosphatase (ThTPase (EC 3.6.1.28)) was revealed in bovine kidney. This form was separated from the major activity peak by ion-exchange chromatography on a DEAE-Toyopearl 650M column, being eluted as a small peak, and purified by a factor of 1472. The both enzyme forms were found to be distinguishable in apparent affinity for substrate, isoelectric point, and activation energy. The comparison of experimental data with theoretical pH values calculated from the amino acid sequence enables us to identify the minor enzyme as a phosphorylated form of ThTPase..
Loiko E. N., Samal A. B.
Influence of H2O2 on ADP-induced platelet aggregation and Ca2+-answer and disaggregation of platelets. pp. 80--83
Summary: In the present study the influence of H2O2 on ADP-induced platelet aggregation and Ca2+-answer is investigated. It is shown that at the presence of a hydrogen peroxide there is a disaggregation of ADP-formed platelet aggregates, its degree depends on concentration H2O2. Under the chosen conditions of experiment (consecutive addition H2O2 and ADP without preincubation of platelets with H2O2) H2O2 did not influence ADP-induced increase of Ca2+-ions, but accelerated their removal from platelet cytoplasm. It is suggested, that the H2O2-dependent removal of Ca2+-ions from platelet cytoplasm is necessary for disaggregation of platelet aggregates.
Nikandrov V. N., Petrusenko G. P., Gronskaya R. I.
The state of ATP-and Ca2+-dependent proteolysis in pheochromocytoma PC12 cells under additions of streptokinase and nerve growth factor. pp. 84--87
Summary: The activity of ATP- and Ca2+-dependent proteases was studied in pheochromocytoma PC 12 cells after their cultivation with plasminogen (SK) and nerve growth factor (NGF) in different concentrations or with combination of SK + NGF. It was found that a short-term exposure of pheochromocytoma PC 12 cells to SK and NGF induced noticeable changes of proteolysis. These changes have complex character, in the dependence on SK and NGF concentrations and combinations. In fact, presented data firstly illustrate the metabolic effect of SK in cells. The mechanism is still unclear, and this phenomen nature is complex task for study in perspective.
Summary: Researches of the last years assume connection of the infection with risk of development of atherosclerosis. To establish a role of Herpes simplex virus of types I and II (HSV1 and HSV2) in pathogenesis of atherosclerosis, 110 patients with clinical attributes of a peripheral atherosclerosis and research of section material from arterial vessels of 28 iclividuals was conducted. It was revealed that in patients with peripheral arterial disease, atherosclerosis of abdominal aorta and odd visceral arteries, showed chronic abdominal ischemia anti is HSV1 + 2 antibody in combination with hyperlipidemia, increase in level of lp(a), associated with fast progressing disease. With increase in duration of disease, frequency of revealing antibodies to HSV1 + 2 grows. Level of fibrinogens is increased in patients infected with HSV1 + 2. By PCR method in atherosclerotic plaques (AP) of the femoral arteries, HSV2 was found. In 64.7% from them, DNA/RNA HSV2 was revealed in leukocytes of blood, antibodies to HSV2 in blood in 86%. In 67% of cases in the abdominal aorta plaques, in 41% -- in the iliac arteries, 72% -- in the superior mesenteric artery, 62% -- in the celiac trunk, HSV2 were found out. There is an interrelation between quantity of arteries, positive to HSV2 and a degree of development of atherosclerotic changes in them. Presence of HSV2 in arteries results in their more expressed stenosis. Our data testify to existence of connection between Herpes simplex virus and peripheral atherosclerosis. Herpes simplex virus of type II related to sites of peripheral vessels, by rich cholesterol and was simultaneously registered in leukocyte blood cells. Therefore, we believe that Herpes simplex virus of type, II individually or together with Herpes simplex virus of type I, can be an initiating agent damaging endothelium and promoting formation of atherosclerotic damages, and hyperlipidemia and or increase in level of lp(a) -- can be a cofactor accelerating atherogenesis.
Morozevich T. A., Semenenya I. N.
Influence of training process on saliva immunoglobulin content in sportsmen. pp. 92--94
Summary: Results of study of saliva immunoglobuhn content of different classes in acrobats before and after training process are presented Possible mechanisms of discovered changes and their importance for evaluation of sportsmen's body state are discussed.
Yermalovich M. A., Samoilovicli E. O., Feldman E. V.
Molecular-biological analysis of polioviruses isolated from patients with vaccine-accociated poliomyelitis in Belarus. pp. 95--101
Summary: Molecular-biological investigation of polioviruses (PV), isolated from children with vaccine-associated poliomyelitis (VAPP) in Belarus in 1967--2002, revealed the high level of their antigenic and genomic variability. For PV type 1 the lost of vaccine strain antigenic properties was predominated. PV type 3 were mainly characterized by recombinant genomes with the part of PV type 1 Among VP type 2 antigenic variability the same as the genomic one were observed. Administration of inactivated polio vaccine for poliomyelitis prophylaxis will allow to prevent the risk of VAPP.
Teplyakov A. I., Prischepova E. V, Akulich N. V., Teplyakova D. V., Kruchinsky N. G.
Role of cytokmes and cell adhesion molecules in pathogenesis of atherosclerosis in patients exposed to low-level ionizing irradiation and living in areas exposed to radionuclide contamination. pp. 102--108
Summary: Mechanisms of intercellular communication disturbances during atherogenesis studies were investigated in 29 patients with atherosclerotic lesions. The research of the initial cytokines (IL-1a, IL-lb, IL-6, IL-8, IL-IO, endothelin-I) and soluble cell adhesion molecules (P-, E-selectins, ICAM-I, VCAM-I) levels and changes in their concentrations in response to coagulation and high shear stress using developed technique are presented. Atherosclerotic lesions of vascular walls were shown to correlate with increased levels of all intercellular adhesion molecules and inflammatory cytokines (IL-lc and IL-6). Distinct elevation of cytokines and bidirectional concentration changes of intercellular adhesion molecules was demonstrated Soluble cell adhesion molecules were proposed to play pathophysiological role in downregulation of cytokines secretion. Thus, proposed interdisciplinary approaches are adequate for research of molecular and cell mechanisms of atherogenesis and worth further development.
Sergeichik N. L., Zhavoronok S. V., Tarasiuk I. V.
Isolation of high antigen activity neurospecific protein S100B and development of immunoenzyme diagnostic set for antibody detection. pp. 109--113
Summary: Fraction of neurospecific proteins S100 with high antigen activity and molecular weight 20.1 kD has been isolated from the brain autopsic tissue (NSP-S100B-20.1). On this basis, new quality IEA for antibody detection has been developed. Its diagnostic sensitivity is 75.7% and diagnostic specificity is 97.8%.
Lobko N. F., Gavrilov V. B., Konev S. V.
Tyrosine-containing peptides -- new indicator of endogenious body intoxication. pp. 114--119
Summary: A new method for diagnostics of endogeneous intoxication (EI) by means of tyrosine-containing peptides (TSP) in blood plasma is developed. The equations for TSP calculation on measurement of UV-absorption at three wave lengths 290, 300 and 305 nm and at one wave length 290 nm (under the simplified formula) are deduced. It is established, that TSP growth brings the basic contribution to increasing total UV absorption of sample at EI. The new indicator TSP is 2.5 times more sensitive than known parameters of toxemia, measured on UV-absorption of deproteinizated plasma (absorption at 210, 254, 280 nm and total absorption in the range of 230--300 nm).
Archakova L. I., Gourine V. N., Yemelyanova A. A., Serduchenko N. S., Novakovskaya S. A.
Effect of the low infensive laser and magnitolaser radiation on the ultrastructure of the damaged subchondral bone. pp. 120--124
Summary: On a model of traumatic arthritis of the knee joint in rabbits, effect of combined blue (l = 441.6 nm) and red (l = 6328 nm) laser radiation and effect of red laser in combination with low magnetic field of 25--50 mTl on the submicroscopic organisation of a surgically damaged repairing subchondral bone at 30 day after operation. It was established that the most favorable effect on the repair of the subchondral bone was produced by 18 session combined treatment of red laser with magnetic field.
Summary: Association of such factors as nonhomologous but cross-reacting with oncotolerogen (OT) of inoculated tumor, OT (IC + tumor-specific complex of free antigens lacking in tumorous cells of sera or tissue of tumor bearer) + adjuvant (egg albumin heterogenous for mice) exhibits antitolerogenic activity and acts as antioncotolerogen that is at immunization of intact organism, induces immunologic reaction directed against oncotolerogen in tumor-bearing organism (oncotolerance) and results in pronounced suppression of inoculated tumor growth (by 118% P < 0.05).
Ogurtsova S. E., Trusova V. D., Krupnova E. V., Voitovich A. M., Afonin V. Yu.
Influence of monooxyge-nase t-BHQ inducer on mutagenic effect of some antitumor drugs in bone marrow cells of mice. pp. 132--134
Summary: Induction of [Ah]-gene battery expression by monofunctional t-BHQ inducer was shown to result in decreasing cytogenic effect of mitomycin C, AZQ and streptonigrin in bone marrow cells of mice C57BL/6J.
Summary: The review deals with the little-studied problem of the involvement of nitric oxide (NO) in the pathogenesis of liver diseases. The available evidence is rather conflicting and demonstrates both hepatotoxic and hepatoprotective effects of NO. At the initial stages of lesion of the liver, the controlled production of NO is accompanied by positive effects of NO, due to its vasodilator and antiapoptotic actions in particular. The formation of conditions, under which the uncontrolled induction of inducible NO-synthase develops, leads, at the background of oxidative stress, to the overproduction of NO and peroxinitrite and, as a result, to necrosis and death of the liver cells.
Feldman E. V., Samoilovich E. O., Votiakov V. I., Titov L. P.
Poliomyelitis in Belarus progress and problems of disease prevention with live oral vaccin. pp. 140--145
Summary: The administration of live oral poliovaccine (OPV) beginning from 1959 has resulted in complete eradication of classical poliomyelitis in Belarus by the end of 60s. In 1967--2002 three imported poliomyelitis cases, caused by wild pohovirus and 53 vaccine-associated paralytic poliomyelitis cases (VAPP), mostly observed among immunocompromised children, were reported Wide-scaled monitoring of polioviruses confirmed the absence of the wild pohovirus circulation in Belarus More than 500 viruses, isolated from different courses had vaccine origin Since 2000 for the prevention of VAPP cases in Belarus, the combined vaccination schedule against poliomyelitis has been used (inactivated polio vaccine is administrated instead of first OPV dose) It is necessary to highly maintain the routine immunization and poliomyelitis surveillance system because the reservoirs of wild pohoviruses still exist in three regions of the wold.