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Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2009, No.4

Homepage / Publications / Scientific Journals

Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2009, No.4

Proceedings of the National Academy of Sciences of Belarus
(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF BIOLOGICAL SCIENCES

Published by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus

Number 4, 2009

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CONTENTS


ARTICLES

Shutova A. G.
Radical scavenger activity of essential oils and entering into their composition terpene and phenolic compounds in various environments. pp. 5--10

Summary: The radical scavenger activity of essential oils and their individual components in relation to ABTS+ has been studied. Differences between antiradical activity in water and alcohol-water environments was found. Quantitative characteristics of essential oils radical scavenger activity are shown to correlate with contents of phenolic compounds.

Salanenka Y. A., Taylor A. G., Laman N. A.
The effect of organic solvents on the perisperm-endosperm envelope permeability of Cucumis sativus seeds. pp. 11--15

Summary: The wax extraction and mechanical alteration are two possible ways in which organic solvents can influence PE envelope permeability have been revealed. The mechanism strongly depends on the nature of the solvents. Non-polar solvents, particularly chloroform, improve the PE envelope permeability by exhaustive extraction of waxes from the envelope. Polar solvents (acetone, methanol, ethanol and pyridine) cause a mechanical alteration breaking the integrity of the cuticular layer of the PE envelope.

Sapegin L. M., Dajneko N. M., Timofeev S. F.
Syntaxonomy of the river Sozh floodplain meadow vegetation in the suburb of Gomel. pp. 16--23

Summary: Syntaxonomy of the river Sozh floodplain meadow vegetation in the suburb of Gomel has been worked out on the basis of long-term (2006-2008) field geobotanic stadies. Prodromus of syntaxons, weather tables of associations' level syntaxons and derivative associations, characterizing tables of 2 neu associations are given. The distinguished associations and derivative associations are related to adequate types of natural forage holdings (NFH). Optimal ecological strategy of use, improvement and protection for every NFH type has been offered.

Islamov R. A.
Chemical modification of lysine and arginine in bifunctional of protein inhibitor a-amilase/ trypsin. pp. 24--27

Summary: Updating lysine and arginine various inhibitors usually spend with the purpose of restriction of their interaction with trypsin. Bifunctional inhibitor a-amylase/trypsin from grain of wheat influenced modifiers lysine citraconic and maleic anhydrides, and arginine 1,2-cyclohexandione and phenylglyoxal. Researches have shown that inhibitor at updating lysine, lost activity in relation to a-amylase mammals whereas change of the rests arginine led to decrease antitryptic to activity bifunctional inhibitor. The received results allow to conclude, that arginine participates in interaction inhibitor with trypsin, whereas lysine with a-amylase.

Kolesneva E. V., Dubovskaya L. V., Volotovski I. D.
Effect nitric oxide on cytosolic free calcium concentration in transgenic Nicotiana plumbaginifolia plants. pp. 28--33

Summary: Effect of nitric oxide donor sodium nitroprusside on cytosolic free calcium concentration ([Ca2+]цит) was revealed. For the first time NO was shown to evoke dose-dependent cGMP-mediated [Ca2+]цит increases. Exogenous NO was found to reduce H2O2 influence on plants decreasing oxidative stress-induced Ca2+-response. The inhibitor analysis showed that endogenous NO responsible for reduction of H2O2-evoked Ca2+-response occurs with the involvement of NOS-like plant enzyme sensitive to oxidative stress.

Bakakina Yu. S., Dubovskaya L. V., Volotovski I. D.
Heat stress modulates endogenous levels of NO and cGMP in Arabidopsis thaliana seedlings. pp. 34--39

Summary: The involvement of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) in heat stress-induced signaling pathway in plants was shown. Heat stress was shown to induce an increase in endogenous content of NO produced by nitrate reductase and NOS-like enzyme and to be followed by the activation of guanylyl cyclase and cGMP synthesis.

Yaronskaya E. B., Averina N. G.
The content and activity of magnesium chelatase in green barley (Hordeum vul-gare) leaves treated with 5-aminololevulinic acid. pp. 40--44

Summary: The Mg-porphyrins has been proposed to play an important role as signaling molecules implicated in the plastid-to-nu-cleus communication. To get more information on the regulation of chlorophyll (ChI) biosynthesis and on Mg-porphyrin functions the content and activity of the key enzyme involved in ChI biosynthesis, magnesium chelatase (MCH), were investigated in the green barley leaves that accumulated excessive amounts of porphyrins from exogenous 5-aminolevulinic acid (ALA) in darkness. Parallel, the content of S-adenosyl-L-methionin:Mg-protoporphyrin IX methyltransferase (MTF), the next enzyme of ChI biosynthesis pathway, as well as the amount of the main protein of the light-harvesting ChI-binding complexes of photosystem 2, Lchbl, were analyzed. The presented data illustrated that the reduction of MCH activity to zero values during the maximum accumulation of MgProto from ALA was due to the considerable decrease of the contents of CHLH and CHLI subunits of MCH. Besides, the decrease of the content of MTF was observed when the accumulation of MgProto reached the maximum level. Our results prove that MgProto is a negative effector of the expression of the nuclear genes encoding CHLI and CHLH subunits of MCH and MTF. The difference between the control and ALA-fed barley leaves in respect to the content of Lhcbl protein was not found, probably because of the various contributions of MgProto and its monomenthyl ester (MgProtoE) in the regulation of the expression of Lhcbl genes. It supports the possible role of MgProtoE as a positive plastid-derived signal. Moreover, the stabilizing effect of ALA on Lhcbl protein could not be excluded. The results obtained testify to the important regulatory role of MgProto in chloroplast-to-nucleus signaling and demonstrate one of the presumed mechanisms controlling the level of accumulation of photodynamic Mg-porphyrin molecules participating in the generation of reactive oxygen species and photodestruction of plant cells.

Domanskaya I. N., Budakova E. A., Samovich T. V., Spivak E. A., Shalygo N. V.
Antioxidant enzymes activity in green barley seedlings (Hordeum vulgare) under water deficit. pp. 45--49

Summary: The effect of soil drought created under laboratory conditions on activity of antioxidant enzyme isoforms, namely ascorbate peroxidase, glutathione reductase, superoxide dismutase and catalase in green barley seedlings (Hordeum vulgare L.) were investigated. The activity of cytosolic and mitochondrial superoxide dismutase isoforms, cytosolic and chloroplastic ascorbate peroxidase isoforms as well as cytosolic glutathione reductase isoform and presumably cytosolic catalase isoform has been shown to increase considerably under water deficit. The data obtained suggests that the expression of genes encoding these enzyme isoforms enhances under soil drought. Genes encoding enzyme isoforms indicated are believed to play an important role in formation of plant response to drought.

Mazhul V. M. †, Shcharbin D. G., Halets I. V., Charnavets T. S.
Phosphorescence of indole and its derivatives in matrix of polyvinyl alcohol. pp. 50--54

Summary: The complex luminescent analysis of tryptophan, indole and their derivatives in solution and matrix of polyvinyl alcohol has been performed. Their spectra of absorbance, fluorescence, phosphorescence at room temperature and phosphorescent lifetimes were measured and the features of structure were discussed.

Chernook T. V., Shcherba V. V., Babitskaya V. G., Smirnov D. A., Ikonnikova N. V., Puchkova T. A.
Medicinal fungi -- lipids producers: cultural and morphological characteristics and biochemical composition. pp. 55--58

Summary: Potential producers of lipophilic compounds Laetiporus sulphureus and Fomesfomentarius (belonging to Basidiomycota), and Cordyceps militaris (belonging to Ascomycota) have been selected among medicinal fungi as the result of the conducted research. The presence of complex of biologically active compounds in their composition allows using them as a valuable raw material for production of medicated products with wide spectrum of activities.

Rakhuba D. V., Novik G. I., Kolomiets E. I., Sidarenka A. V., Belyasova N. A.
Growth and morphology of microorganisms Bifidobacterium genus in anaerobic station Bug Box M. pp. 59--64

Summary: The growth and cell morphology at anaerobic fermentation of bifidobacteria on the agar surface were studied. Anaerobic atmosphere contained hydrogen -- 9%, carbon dioxide -- 10%, nitrogen -- 81%. Intense growth was shown in presence of studied gas mixture. The formation of the bifidobacteria cell forms typical for normar population development was observed. These form contained budding rod-shaped and coccal forms, rod-shaped cells with terminal bifurcation and square-headed swelling, branching filaments.

Kuis L. V., Markevich R. M., Lajkovskaja I. V.
Influence of nutrient medium composition to accumulation of organic acids by bacteria of the genus Bacillus. pp. 65--69

Summary: The purpose of work was determination of organic acids in cultural liquids of bacteria Bacillus mucilaginosus N 4 and Bacillus sp. G grown on synthetic and natural nutrient mediums. Methods of TLC and GLC were used for analysis, the technique of test cultural liquids preparation for GLC analysis was advanced. Presence in all studied cultural liquids of the following organic acids was established: volatile (formic, acetic); non-volatile: monocarboxylic (lactic, pyruvic), dicarboxylic (oxaloacetic, oxalic, succinic, tartaric), tricarboxylic (citric). The tartaric acid was found out in cultural the liquids obtained on synthetic mediums.

Ryabzeva T. V., Kapich A. N.
Peculiarities of the process of lipid peroxidation during phagocytosis of microorganisms with different structure of cell wall. pp. 70--75

Summary: The aim of this work was to investigate the activity of lipid peroxidation (LPO) during phagocytosis of different prokaryotic and eukaryotic microorganisms by neutrophils and to estimate correlation between the microbicidal activity of phagocytes and LPO. The activation of LPO was found during phagocytosis of all tested microorganisms. The highest level of TBA-active products of LPO was accumulated in the course of phagocytosis of gram-negative bacteria (E. coli). In this work we for the first time show that the activation of LPO developed during phagocytosis of microorganisms can contribute to microbial killing. The results of our study indicate that along with the damaging impact, activation of LPO may have important positive role in phagocytosis.

Kravchenko E. V., Maksimova L. V.
Influence of zoosocial factor on habitutation processes in inbred mice BALB/c and C57B1/6 with different anxiety levels. pp. 76--81

Summary: The ability to nonassociative learning either in absence or in presence of zoosocial interactions is reduced in "timid" mice BALB/c in comparison to C57B1/6. In these conditions intraspecies communications significantly improve habituation of locomotion and rearings either in pathologically high anxious BALB/c mice, or in C57B1/6.

Ivanova S. V., Kirpichonok L. N.
Own fluorescence of human serum albumin and products of its proteolysis. pp. 82--88

Summary: The study, of fluorescence spectra of human serum albumin (HSA) has shown, that proteolysis of HSA under action of trypsin is accompanied by change of parameters of own fluorescence of proteins. The obtained data can be used for study of different enzyme-substrate interaction on the base of changing of own fluorescence of proteins.

Zhurikhina L. N.
Complex biological estimation of the influence of multicomponent FDS on the growth of infusorium Tetrahymena periformis. pp. 89--95

Summary: A complex biological estimation of the four formulations of the food dietary supplements (FDS) differing in combinations of their structural vegetative and reproductive organs of Rosaceae, Berberidaceae and Magnoliceae plants has been carried out using the test-system Tetrahymena pyriformis. The dependences "doze-time-effect" in the range of supersmall, small and toxic concentrations has been examined. The combined effect of the components of FDS has been estimated. The protective properties of the food dietary supplements during the isolated, combined and associated influence of the lead nitrate, sodium nitrite the ultraviolet radiation have been investigated. The optimum alternatives of the formulations of FDS have been selected. The toxically safe and protectively effective dosages have been grounded.

Denisova S. I.
Peculiarities of utilizing foodstuff mineral elements by Antheraea pernyi in experimentally weakened plants. pp. 96--103

Summary: In the initial stage of experimental weakening (24--48 hours of storing cut leaves of Quercus robur L., Betula pendula Roth, and Salix viminalis L.) the quality of Antheraea pernyi G.-M. feeding plants is improving because of increase in soluble carbohydrates, free amino acids in leaves and digestion of calcium, potassium, phosphorus, magnesium, and copper rises were shown. In the closing weakening stage (72 hours) the quality of feeding plants deteriorates because of efflux of soluble carbohydrates, free amino acids, calcium, potassium, and phosphorus from leaves to stems, accumulation of copper and digestion of calcium, potassium, phosphorus, magnesium, and copper decreases.

Alekhnovich A. V.
The present spreading of spiny-cheek crayfish Orconectes limosus in Belarus and the peculiarities of its life cycle. pp. 104--110

Summary: An invasive species -- spiny-cheek crayfish Orconectes limosus (Rafinesque, 1817) has widened its spread since 1997, before that it was encountered only in the far north-western part of Belarus in the basin of River Neman in Grodno region. Currently it is encountered in the rivers of Belovezhskaya Pushcha and head waters of River Neman region in River Izled (Nalibokskaya Pushcha).

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SHORT COMMUNICATIONS

Bashilov A. V.
Phytochemical composition and pharmacological properties Filipendula hexapetala. pp. 111--114

Summary: Results of researches in the field of pharmacology and phytochemical composition Filipendula hexapetala Gilib. are presented.

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SCIENTISTS OF BELARUS

Victor Ivanovich Parfenov (To the 75th Anniversary). pp. 115--118
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here to read this article in Russian (Win1251)

Igor Dmitrievich Volotovski (To the 70th Anniversary). pp. 119--121
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Uladimir Kirillovich Sauchanka (To the 70th Anniversary). pp. 122--123
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