Изв. Нац. академии наук Беларуси. Сер. мед. наук, 2005, No.1

Изв. Нац. академии наук Беларуси. Сер. мед. наук, 2005, No.1

Известия Национальной академии наук Беларуси
СЕРИЯ МЕДИЦИНСКИХ НАУК
Издатель Беларуская навука, Минск, Республика Беларусь

Номер 1, 2005

СОДЕРЖАНИЕ

КЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНА

Beloenko E. D., Eismont O. L., Pashkevich L. A., Shved I. A., Ermakov S. F.
Effectiveness of medication containing bioactive cholesteric-nematic liquid crystals substance in treatment of experimental osteoarthritis. pp. 5--8

Summary: The purpose of this study is to evaluate the use of medication containing cholesteric-nematic liquid crystals substance in treatment of experimental osteoarthritis in rats. We have concluded that studied medication is very effective modificator of synovial fluid and articular cartilage properties. The effects of the medication consist in decrease in dystrophic changes and increase in biosynthetic processes in articular cartilage, cartilage protection from enzymatic and mechanic destruction.

Shanko Y. G.
Diagnostics and surgical treatment of meningiomas of the pontocerebellar angle. pp. 9--14

Summary: The analysis of results of surgical treatment of 66 cases of cerebello-pontine angle meningiomas is present. All of them are operated on through the unilateral suboccipital retrosigmoid approach. Radical removal of tumor (Simpson 2 and 3) was made in 53--80.3% cases, partial (Simpson 4) -- in 13--19.7% cases. Early postoperative period is characterized by neuropathies of cranial nerves and cerebellar ataxia. Postoperative mortality was 4.5% (3 cases). Late results were studied in 42 patients. Quality of life by Karnovsky scale in 7--16.7% cases was 100--90%, in 19--45.2% cases -- 80--70%, in 15--35.7% cases -- 60--50% and only in 1--2.4% cases -- 40--30%.

Zarovskaya A. V., Beloenko E. D., Korzun O. A., Kulchitsky V. A.
Analysis of causes of regional hemodynamics dysregulation following malleolus trauma. pp. 15--19

Summary: The results of the electrophysiological research of the regional blood flow in lower limbs (rheography, color duplex sonography) were submitted in 31 patients with severe malleolus trauma in early and late periods after surgical treatment and in 30 healthy volunteers as a control. It was shown that at an acute period after trauma the phase of the vasoconstriction in the period of 1--4 days replaced the phase of vasodilatation due to many factors, which included the actions of humoral and local autoregulatory mechanisms (due to tissue ischemia) and the weakening of the excitatory influences as a result of the injury of the fibres, initiation of the endothelium depending mechanisms. Seven to ten days after osteosynthesis operation there was a trend to increasing in the blood vessel tonus, which was the same at the period of 1--3 months. 8--12 months after the operation, the blood circulation of the shin was repaired, but the blood vessels spasm of the injured foot was retained.

Bychkov A. V., Semenenya I. N., Serdyuchenko N. S.
Dependence of duration of postoperative hospital stay of children on time of the day in case of appendectomy operations (by the example of acute gangrenous appendicitis). pp. 20--25

Summary: The dependence of the index of the hospital treatment duration of children after operations on the grounds of acute gangrenous appendicitis on the moment of appendectomy in the day and night cycle has been identified and described by the mathematical model. Children were recovering after stated operations performed during the night and early morning time more slowly than after the same performed during the rest of the time.

Pesotskaya Y. A., Netukova N. I., Slinko S. K., Pashkevich S. G., Koulchitsky S. V., Yemelyanova A. A., Antipenko A. A., Kurek V. V., Kulchitsky V. A.
Cytoarchitectonics of segmental and suprasegmental spinal and brain regions in different periods of observation after high thoracic epidural anesthesia. pp. 26--30

Summary: The infrastructure of spinal cells (Th3-Th4) and spinal roots at the level of application of anesthetics (bupivacain 1 mg/kg and calipsol 0.5 mg/kg), spinal segments located caudal to Th3-Th4 (Th6-Th7), and suprasegmental regions of the nervous system (raphe nuclei of the medulla) was compared in different periods (up to 2 weeks) after high thoracic epidural anesthesia. Apparent alterations of smooth muscle cells in microvessels, nerve cell processes and gliocytes in the spinal cord mainly at the level of anesthetic application were found.

Baranovsky A. E.
Hyperbaric oxygenation in correction of postoperative neurological disorders in patients with meningiomas of the wings of the basilar bone (MWBB) and the cavernous sine. pp. 31--34

Summary: Results of application of hyperbaric oxygenation (HBO) in the early postoperative period on patients who had the operation for removing the meningioms of the wings of the main bone (MWMB) and the cavernous sine are presented. The effectiveness of the applied method is shown as an addition to the routine therapy (52.9% of excellent and good results in patients who received a course of HBO against 44% without HBO). Application of HBO in the early postoperative period on patients with MWMB makes it possible to lessen the time of patient stay in hospital as well as to reduce temporary disability among the presented group of patients.

Parkhach L. P., Titovets E. P.
Tissue oxygenation in conditions of aquaporin channel inhibition. pp. 35--37

Summary: The effect of aquaporin inhibition on parameters of oxygen mass transfer in various tissues and tissue membranes of rabbits was investigated. There were demonstrated high steady-state levels of oxygen partial pressure on the brain surface, parietal peritoneum, pericardial sac and corresponding body fluids. There was demonstrated a decrease in the oxygen mass transfer rates through the tissue membrane after exposure to aquaporin inhibitors. The experimental results support the concept of the microaquacirculatory mechanism of tissue oxygenation.

Shnitko S. N., Strinkevich A. L.
Pathomorphological changes in neurons in thoracic sympathetic ganglia in patients with Raynaud's phenomenon and in experiment. pp. 38--40

Summary: We have studied character and distinctive features of neuronic infringements at Raynaud's disease and experimental cooling and ischemia of forward extremities of the rabbits. The reduction of the sizes of neurones is a prevailing type of alterative infringements at Raynaud's disease. Intensifying the alterative and subsequent destruction of neurones is proved at worsening of the disease. Hemodynamic effect of sympathectomy at Raynaud's disease is minimal at the expressed alterative of neurones in sympathetic ganglions. The quantity of neurones with the diminished sizes was less in the rabbits after experiment, than the patients with Raynaud's disease. We confirm, on the basis of results of experiment, that cooling and ischemia of extremities are not the reason of alterative destruction of neurones in sympathetic ganglions (decrease of the sizes of neurones) at Raynaud's disease.

Titov P. L., Naumovich S. A.
Immune system parameters in patients with complaints of adverse effects of dental alloys. pp. 41--48

Summary: Most fixed and removable dentures are made from casting alloys. Many orthodontic appliances are also fabricated from metallic biomaterials. It has been documented in vitro and in vivo that metallic restorations release metal ions mainly due to corrosion. Those metallic ions may be distributed systemically and locally and could pay a significant role in the induction of oral or/and systemic immunoinflammatory conditions. The aim of the present study was to study the phenotypic and functional activities of peripheral blood mononuclears from patients with complaints suspected to adverse effects of dental alloys. We examined 31 patients (29 women and 2 men) aged 35--71 years with symptoms assumed to adverse effects of dental alloys. All patients were studied with base metal salts (Cu²⁺, Co²⁺, Cr⁶⁺, Mg²⁺, Mn²⁺, Ni²⁺, Ti³⁺, Zn²⁺) for patch tests. 21 healthy people served as control subjects. Phenotyping of peripheral blood mononuclears was assessed by indirect immunofluorescence staining after incubation with monoclonal antibodies specific for CD3⁺, CD4⁺, CD8⁺, CD 11b⁺, CD20⁺, CD25⁺, CD45⁺, CD95⁺ and HLA-DR⁺ by flow cytometry. Number of CD20⁺-cells slightly reduced (9.29+1.5). The total count of T-lymphocytes reduced insignificantly (60.75+3.36), as well as CD4⁺-cells (37.03+2.61). Decrease in CD8⁺-cells has been more expressed at sensitized to nickel (19.43±2.03) and cobalt (12.47±2.16) patients and at the patients with objective symptoms (18.42±2.39). Significant rise in CD4⁺ (T-helpers) and CD8⁺ (T-supressors) ratio was found in patients with positive patch tests to nickel (2.35±0.4) and cobalt (4.09±1.04) (P < 0.05). Reliable variations of expression of activation (CD25⁺, CD45⁺, CD11b, HLA-DR) and apoptosis (CD95⁺) markers were revealed in sensitized to metals patients (P < 0.05). Obtained results reveal more significant changes in T-lymphocytes (CD3⁺, CD4⁺, CD8⁺) subpopulations and expression of activation molecules confirming primary role of T-lymphpcytes in development of such oral immunopathological conditions.

Smeyanovich A. F., Shanko Y. G., Khodulev V. I.
Aspects of facial nerve preservation in surgery of posterior cranial fossa parastemal tumors. pp. 49--54

Summary: The efficiency of carried on to preserve the facial nerve anatomic safety of removal of posterior cranial fossa parastemal tumors is analyzed in 463 cases. The anatomic safety of facial nerve has been preserved in 245--72.9% cases of acoustic schwannomas of III--IV degree, in 81--81.0% cases of parastemal meningiomas, in 5--71.4% cases of trigeminal schwannomas and in all cases of jugular schwannomas and parastemal cholesteatomas. The degree of functional status according to the J. W. House, D. E. Brackmann scale was estimated in 253--54.6% cases after the postoperative period of one year. Excellent and good results (HB 1 -- HB 2) were achieved in 150--59.3% cases, satisfactory (HB 3 -- HB 4) -- in 39--15.4% cases, poor results or no recovery (HB 5 -- HB 6) -- in 64--25.3% cases.

Nechipurenko N. I., Sidorovich E. K., Antonov I. P., Vasilevskaya L. A.
Evaluation of magnesium sulfate and glycine efficiency in patients with brain infarction. pp. 55--58

Summary: The magnesium sulfate and glycinum efficiency has been studied in acute period of brain infarction. There were formed 3 patient groups for this purpose. The patients of the 1^st group (21 persons) were given glycinum, the patients of the 2^nd one (20) -- magnesium sulfate with medicines of standard therapy. The control group consisted of 18 persons who were given standard therapy only. The dermal microhaemodynamics status was investigated by speckle-optical method. It has been established the positive influence of glycinum and magnesium sulfate in the complex treatment of cerebral infarction. However more marked effect was revealed in neurological status estimation of patients with magnesium application. It is expedient for primary neuroprotection to use the antagonists of NMDA-receptors, for example, magnesium ions. It has been shown the informative capacity of presented parameters for estimation of dermal microhaemodynamics status with speckle-optical method in examined patients.

Slobozhanina E. L, Zubritskaja G. P., Antonovich A. N., Kozlova N. M., Kozarezova T. L, Klimkovich N. N., Bartosz G.
Activity of glutathione-S-transferase and output of glutathione conjugates from erythrocytes in children with aplastic anemia and myelodisplastic syndrome. pp. 59--63

Summary: Comparative research of activity cytoplasmic glutathione-S-transferase (GST) and export glutathione-S-conjugates (DNP-SG) from erythrocytes in children with aplastic anemia (AA) and myelodisplastic syndrome (MDS) and practically healthy children was carried out. It was shown that in erythrocytes of the children suffering from AA and MDS, the activity of GST was in 1.8--2 times higher than activity of enzymes in erythrocytes practically healthy children. The output of DNP-SG-conjugates occured much more intensively from erythrocytes of the children, suffering from AA and MDS, in comparison with erythrocytes of practically healthy children. The data received in the present research allow to consider that the increased transport of glutathione-S-conjugates from erythrocytes of the children suffering from AA and MDS is probably a consequence of raised speed of their formation that occurs at participation of GST. Moreover, increase in activity of cytoplasmic glutathione-S-transferase and increase in speed of export of glutathione-S-conjugates from erythrocytes of the children suffering from AA and MDS can be connected to peculiarities of conjugation of GSH and CDNB, and to modification of a physico-chemical state of erythrocytes membranes.

Komar S. N., Sidorenko V. N.
Prognosis of risk of thrombotic complications in obstetrical practice. pp. 64--67

Summary: The information was obtained using a prognosable table of risk degree of should be used thrombotik complications compared with the information obtained in a laboratory. The prognosable table should be used as criterion of women selection, in group of risk of thrombotic complications, with a gene for prevention of this pathology.

Mitkovskaya N. P., Taghyzadeh G. H., Mansurov V. A., Kolyadko M. G., Levin V. I., Kaminskaya T. V., Grigorenko E. A., Yanovich E. A., Chyzh A. K.
Metabolic syndrome: parameters of microcirculation and peripheral and central hemodynamics in patients with ischemic heart disease and diabetes of type II. pp. 68--75

Summary: It has been revealed that the combination of ischemic heart disease (IHD) and diabetes mellitus (DM) of type II is accompanied by microcirculation bed Theological component parameters change in patients older than 40 that correlates with insulin and hydrocortisone concentration in contrast to such parameters in patients with IHD when similar changes were determined in patients older than 60. The combination of IHD and DM of type II is accompanied by changes in plasma viscosity, diabetic retinopathy of stage III in 100% of patients older than 40 without dependence on IHD course; by development of diabetic retinopathy of stage III symptoms in patients older than 60; by vessels endothelium dysfunction (ED) of brachial artery 3.3 times more often in comparison with IHD patients group; by diastolic dysfunction aggravation of the left and right ventricle. The combination of IHD and DM of type II is characterized by reliable increase in total cholesterol, triglycerides, lipoprotein (a) concentrations, and decrease in high-density lipoproteins level that correlates with ED; by increase in pre-p-lipoprotein concentration and decrease in dehydroepiandrosterone concentration that correlates with ED and hydrocortisone concentration; by increase in A24 (9) and Bw61 (40) antigens occurrence and decrease in antigen Cw4 occurrence in comparison with patient group with ischemic heart disease.

Mastykin A. S., Drivotinov B. V., Apanel E. N.
Multivariate statistical estimation of transient ischemic attacks heterogeneity. pp. 76--82

Summary: The data of own researches on heterogeneity of nosology concept of transient ischemic attack (TIA) are given. With the use of the discriminant functional analysis, etiopathogenetic heterogeneity of this nosology structure which is formed by atherosclerotic, cardioembolic, and arterial hypertensive components is proved. Borders between these sets are indistinct. These sets as mathematical multitudes are detached enough, but at the same time, are crossed with each other. Such parity is caused by the proved etiopathogenetic variety of this disease with involving in pathological process of several structural systems of the organism -- it is the basis of the TIA heterogenesis concept. Proceeding from this concept, practical recommendations for prevention of this pathology and its relapses are given.

Voitovich A. M., Trusova V. D., Krupnova E. V., Ogurtsova S. E., Afonin V. Yu.
Micronuclei in lung cells of C57BL/6J mice after mitomycin C treatment. pp. 83--86

Summary: An acute experiment with mitomycin C has shown that in C57BL/6J mice the maximum level of alveolar macrophages and type II alveolocytes (pneumocytes) with micronuclei was observed 42 h after exposure. Two peaks of yield of alveolar macrophages with micronuclei were observed 18 and 42 h after mitomycin C treatment by preliminary administration of b-naphthoflavone.

Titov P. L., Kondrachuk A. N.
Study of nickel genotoxicity in mononuclears of peripheral blood by Fast Micromethod®. pp. 87--91

Summary: Nickel is used for a variety of applications including removable partial denture frameworks, components of appliances in orthodontics and pediatric dentistry and crowns and fixed bridges. It has been documented in vitro and in vivo, that metallic restorations release metal ions mainly due to corrosion. Those metallic ions may be distributed systemically and locally and could pay a significant role in the induction of oral or/and systemic pathological conditions. Our purpose was to study the nickel sulphate genotoxicity in mononuclears of peripheral blood depending on concentration and time of exposition. Single strand DNA breaks of the peripheral blood mononuclears from 21 donors were determined by Fast Micromethod to different concentration (50, 100, 250 and 500 mM) of NiSO₄ and time (3 and 6h) of exposition. The method is based on the ability of the specific fluorochrome dye PicoGreen to make a very stable complex with ssDNA in highly alkaline conditions. PBMs (3000 cells/25 c) were lysed by special lysing solution (9 M urea, 0.1% SDS, 0.2 M EDTA, pH10) supplemented with PicoGreen. DNA denaturation followed directly after addition of 250 c of NaOH (pH = 12), for 20 min, in the microplate by measuring the fluorescence (excitation 485 mn/emission 520 nm) of the ssDNA-PicoGreen complex by Fluoroscan, Labsystem. Results were expressed as strand scission factors (SSFs), calculated as the log10 of the ratio of the percentage of ssDNA from treated and control samples, respectively, after 20 min of denaturation. For practical reasons strand scission factors were multiplied by -1. Our results showed that Fast Micromethod used for the measurement of DNA damaging and genotoxicity assesment as well as biomonitoring of the negative effects of genotoxins on cells of the immune system. Ni²⁺ ions induced DNA damage depends on concentration and time of exposition. Moreover, there is an individual variability of genosensitivity/genoresistance in population to the same concentarations of genotoxins.

Vinogradov V. M., Kukhareva L. V., Eliashevich A. V.
Toxicological properties of Melittis sarmatica Klock. extracts after single dosing to hybrid (CBA C57BL6) F1 mice. pp. 92--95

Summary: Intoxicants for mice doses of water-ethanol extracts for herb of the Melittis sarmatica Klock. have been established in clinical investigations.

ОБЗОРЫ

Semenenya I. N.
Golden rule of scientific thought. pp. 96--103

Summary: Analysis of reference state rule known in literature as the "Leites phenomenon" is presented in this paper. It is shown that the rule, which can be considered the golden rule of scientific thought, is a basic methodological principle of analysis of all natural phenomena and processes in addition to separate manifestations of life as shown by the professor S. M. Leites in the field of pathophysiology of metabolism and nervous and endocrine systems. Necessity of further development and use of this rule, as a required tool harmonizing the scientific thought, in scientific effort is shown.

Pashuk V. P.
Experiment on development of biotechnology for production of antigenic drugs for immunologic studies in treatment of intestinal and tissue helminth invasions. pp. 104--115

Summary: The materials on the experience of biotechnology development for production of antigenic and serum helminth drugs for use in the serum diagnostics of the epidemically hazardous intestinal tissue invasion of trichinosis, cysticercosis and ascaridiasis of mono- and miscellaneous contagion. The stated methods are used for differential- and diagnostic purposes for detection of tissue invasion among community with the similar clinical course of parasitogenic and infectious diseases of bacterial and viral ethiology. In the view of the existing parasitogenic situation, it is recommended to organize a consultative and methodical centre for prevention and treatment of the above-stated invasion on the basis of the long-term experience.

Kizyukevich L. S., Kizyukevich I. L.
Analysis of complications in internal organs after surgical interventions in biliary tract. pp. 116--124

Summary: This survey showed that the surgical interventions in biliary tract may be accompanied by serious multiorgan disturbances, sometimes with a fatal outcome. The deep analysis of the causes for such complications and the preventive ways recommended will allow to decrease a complication frequency and amount of lethal outcomes and thereby to rise the quality of treatment in patients with biliary tract pathologies.

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Разработана и поддерживается Николаем Н. Костюковичем. Последнее обновление: 5 июня 2005 г.
Создана при участии Игнатия И. Корсака
Копирайт © 2005 Национальная академия наук Беларуси
Копирайт © 2005 Издательство "Беларуская навука"